Simultaneous identification of multiple receptors of natural product using an optimized cDNA phage display cloning

Qing-Li He; Hui Jiang; Feng Zhang; Hai-Bao Chen; Gong-Li Tang

doi:10.1016/j.bmcl.2008.06.006

Simultaneous identification of multiple receptors of natural product using an optimized cDNA phage display cloning

Bioorg Med Chem Lett. 2008 Jul 15;18(14):3995-8. doi: 10.1016/j.bmcl.2008.06.006. Epub 2008 Jun 6.

Authors

Qing-Li He¹, Hui Jiang, Feng Zhang, Hai-Bao Chen, Gong-Li Tang

Affiliation

¹ State Key Laboratory of Bio-organic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 354 Fenglin Road, Shanghai 200032, China.

PMID: 18571405
DOI: 10.1016/j.bmcl.2008.06.006

Abstract

Simultaneously isolating more than one receptor of natural product remains a challenge to chemical genetics. Using cyclosporine A as an affinity probe and an optimized phage display cloning procedure, not only cyclophilin A, but also cyclophilin B was isolated as the full-length gene clone from a human brain cDNA library. This optimized protocol can be used to select protein targets of chemicals dependent on the binding affinity rather than on the relative abundance in cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteriophages / metabolism
Biotinylation
Brain / metabolism*
Chemistry, Pharmaceutical / methods
Cloning, Molecular
Cyclophilin A / chemistry
Cyclophilins / chemistry
DNA, Complementary / metabolism
Gene Library*
Humans
Kinetics
Models, Chemical
Oligonucleotide Array Sequence Analysis / instrumentation*
Oligonucleotide Array Sequence Analysis / methods*
Peptide Library
Technology, Pharmaceutical / methods

Substances

DNA, Complementary
Peptide Library
cyclophilin B
Cyclophilin A
Cyclophilins